“Paradigm Shifts: Impacts on Science” – Amber Mosley @ Cafe Inquiry

Mosley, Cafe Inquiry Co-hosted by Center for Inquiry - Indy and Central Indiana Science OutreachPUBLIC LECTURE, JUNE 24th, 7pm CENTER FOR INQUIRY, DOWNTOWN INDIANAPOLIS

CINSO and the Center for Inquiry of Indiana is co-hosting the monthly lecture series “Cafe Inquiry.”

Throughout the history of science, there have many instances in which experimental evidence requires society to make a fundamental change in underlying assumptions. In this discussion Dr. Amber Mosley will discuss the concept of a paradigm, which can be defined as a set of practices, models, and ideas that define a scientific discipline at a specific point in time. During her lecture, Dr. Mosley will discuss a previously held paradigm known as the Phlogiston Theory (the theory that a fire-like element called phlogiston was contained within combustible substances and released when burned) and compare it to the modern day paradigm shifts regarding the Central Dogma of Molecular Biology and repression of transcription. Current work in the Mosley Lab supporting these new paradigm shifts will be shared as well.

Mosley received her Bachelor’s Degree in Biology and Philosophy from East Tennessee State University, her Ph.D. in Biochemistry from the University of Kentucky College of Medicine, and was a Postdoctoral fellow at the Stowers Institute for Medical Research. She joined the Indiana University School of Medicine as an Assistant Professor in 2010, studying the fundamental biological process of RNA transcription.

In multiple disease states ranging from cancer to viral infection, the mechanisms of general transcription are altered. In order to understand the extent of disease manipulation of the transcription machinery, general mechanisms that control transcription under normal conditions must be understood. The Mosley Lab applies quantitative proteomic and genomic techniques to study the dynamics of RNA Polymerase II transcription organism-wide. Using Multidimentional Protein Indentification Technology (MudPIT), the lab studies both protein-protein interaction networks and post-translational modifications that may alter transcription during different cellular states. In parallel, using approaches such as RNA sequencing and DNA interaction analysis (known as ChIP-sequencing) the Mosley lab can study the effects of genetic and environmental changes on gene transcription in vivo.